Ingestion of Ice-nucleating Active Bacteria Increases the Supercooling Point of the Lady Beetle Hippodamia Convergens
نویسنده
چکیده
Regulation of the temperature at which insects freeze is a critical factor in their overwintering survival. Although the role of ice-nucleating active bacteria in promoting frost-damage in plants is well known, their interaction with other organisms has not been described. In winter. many freeze-susceptible insects avoid freezing by lowering the temperature at which their body fluids spontaneously nucleate, termed the supercooling point. The lady beetle, Hippodamiu conuergens (Coccinellidae, Coleoptera), is a freeze-susceptible insect that overwinters as an adult in the supercooled state, 16°C. In the control group, none of the beetles fed only water had supercooling points above lS”C, similarly no beetles fed an ice-nucleating negative bacterium had supercooling points above 14°C. Following ingestion of the ice-nucleating active bacteria, Pseudomonas syringue and Erwinia herbicolu ( IO9 bacteria/ml), the supercooling point was elevated to -3.5 and -4.4”C respectively, the supercooling point remained elevated for 7 days after P. syringae treatment and a dose-response relationship was observed. Key Word Index: Pseudomonas syringae; Erwinia herbicola; Hippodamia convergens; supercooling point; ice-nucleating agents
منابع مشابه
Topical application of ice-nucleating-active bacteria decreases insect cold tolerance.
The majority of overwintering insects avoid lethal freezing by lowering the temperature at which ice spontaneously nucleates within their body fluids. We examined the effect of ice-nucleating-active bacteria on the cold-hardiness of the lady beetle, Hippodamia convergens, a freeze-intolerant species that overwinters by supercooling to ca. -16 degrees C. Topical application of the ice-nucleating...
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تاریخ انتشار 2003